Efficient and Fast Purification Method for Fluorescent Dye-Labeled Oligonucleotides
نویسندگان
چکیده
منابع مشابه
A convenient and efficient purification method for chemically labeled oligonucleotides.
We developed an efficient, cost-effective, and rapid purification method for chemically-labeled oligonucleotides that requires less time than conventional procedures such as ethanol precipitation or size-exclusion chromatography. Based on the hydrophilic and hydrophobic properties of DNA and amine-reactive fluorophores, we show that n-butanol saturated with distilled water may be used to remove...
متن کاملAn Optimized Protocol for Electrophoretic Mobility Shift Assay Using Infrared Fluorescent Dye-labeled Oligonucleotides.
Electrophoretic Mobility Shift Assays (EMSA) are an instrumental tool to characterize the interactions between proteins and their target DNA sequences. Radioactivity has been the predominant method of DNA labeling in EMSAs. However, recent advances in fluorescent dyes and scanning methods have prompted the use of fluorescent tagging of DNA as an alternative to radioactivity for the advantages o...
متن کاملGeneral and facile purification of dye-labeled oligonucleotides by pH-controlled extraction.
Previously, we reported a method for facile purification of oligonucleotides labeled with hydrophobic dyes, based on the solubility difference between the hydrophilic DNA and unreacted dye. Here, we present a new purification method applicable to any dye regardless of its hydrophobicity. We exploited the population shift of a fluorescent dye in a low-pH aqueous solution from its anionic form to...
متن کاملData on single-step purification method for dye-labeled DNA sequencing
Dye-labelled DNA sequencing is one of the most common and robust technique required for molecular biology since 1977 (Sanger, 1977) [1]. I have recently provided the single-step purification method for dye-labeled sequencing products, which is based on the removal of the washing step in EDTA/ethanol precipitation (Fujikura, 2015) [2]. Here I assess and report the accumulated data of the modifie...
متن کاملRapid purification of fluorescent dye-labeled products in a 96-well format for high-throughput automated DNA sequencing.
Large-scale DNA sequencing projects can be limited by various technical bottlenecks. As more DNA templates are generated and successfully processed, the mechanisms and technologies continue to be improved and optimized. Fluorescence-based automated sequencing (1) has replaced radioisotope-based manual DNA sequencing (2) as the primary approach in large-scale genome projects. For this approach, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2018
ISSN: 0006-3495
DOI: 10.1016/j.bpj.2017.11.3676